李月, 余泽林, 王瑞白. 嗜麦芽窄食单胞菌标准株的建立及评价[J]. 凯发娱乐加盟代理, 2023, 38(12): 1484-1490. DOI: 10.3784/jbjc.202311120496
引用本文: 李月, 余泽林, 王瑞白. 嗜麦芽窄食单胞菌标准株的建立及评价[J]. 凯发娱乐加盟代理, 2023, 38(12): 1484-1490. DOI: 10.3784/jbjc.202311120496
Li Yue, Yu Zelin, Wang Ruibai. Establishment and evaluation of a reference strain of Stenotrophomonas maltophilia[J]. Disease Surveillance, 2023, 38(12): 1484-1490. DOI: 10.3784/jbjc.202311120496
Citation: Li Yue, Yu Zelin, Wang Ruibai. Establishment and evaluation of a reference strain of Stenotrophomonas maltophilia[J]. Disease Surveillance, 2023, 38(12): 1484-1490. DOI: 10.3784/jbjc.202311120496

嗜麦芽窄食单胞菌标准株的建立及评价

Establishment and evaluation of a reference strain of Stenotrophomonas maltophilia

  • 摘要:
    目的 对1株嗜麦芽窄食单胞菌中国临床分离株进行系统性评价,建立嗜麦芽窄食单胞菌标准株。
    方法 参照《病原微生物菌(毒)种国家标准株评价技术标准》(WS/T 812-2022),对嗜麦芽窄食单胞菌中国分离株11066进行形态、运动性、培养条件、生化反应、药物敏感性、质谱等检测以及16S rRNA测序、多位点序列分型分析及全基因组测序等分子生物学检测,并对该菌株进行扩大培养,建立主代种子库及工作菌株库。
    结果 菌株11066为革兰阴性杆菌,电镜下可见1~4根极端鞭毛;在血平板和其他营养琼脂上生长良好,生长条件为15~37 ℃、pH 5~10和0~5%盐浓度;动力阳性;硝酸盐还原试验阳性、水解七叶灵和明胶试验阳性;生长可利用葡萄糖、甘露糖、N-乙酰-葡萄糖胺、麦芽糖、柠檬酸和苹果酸为碳源;对阿米卡星、头孢西丁等18种革兰阴性菌常用抗生素耐药;主代种子库和工作菌株库菌株生物学性状稳定,传代等信息完整;菌株已保藏于中国疾病预防控制中心病原微生物菌(毒)种保藏中心并获得国家保藏编号。
    结论 嗜麦芽窄食单胞菌中国临床分离株11066有明确的来源信息,具有典型的嗜麦芽窄食单胞菌生物学及遗传学特征,已按国家标准株评价标准进行评价并保存于公认的来源/保藏中心,可以作为标准株/参考株应用于临床病原微生物检测质控和基础科研。

     

    Abstract:
    Objective To systematically evaluate the Chinese clinical strain 11066 and establish a reference strain of Stenotrophomonas maltophilia in China.
    Methods According to the Technical Requirements for Evaluation of National Standard Strains of Pathogenic Microorganisms(WS/T 812-2022), the morphology, motility, culture conditions, biochemical reactions, drug sensitivity, mass spectrometry of the Chinese S. maltophila isolate 11066 were tested, as well as 16S rRNA sequencing, multilocus sequence typing analysis, and whole genome sequencing. The strain was expand cultured and libraries of main seed and working strain were established.
    Results Strain 11066 is a Gram negative bacterium, with 1–4 extreme flagella visible under electron microscopy. It grows well on blood plates and other nutrient agar under conditions of 15–37 ℃, pH 5–10, and 0–5% salt concentration. It was motivation positive, has positive results in nitrate reduction test, hydrolysis heptyl and gelatin test and can utilize glucose, mannose, N-acetylglucosamine, maltose, citric acid, and malic acid as carbon sources. It was resistance to 18 commonly used antibiotics against Gram negative bacteria such as amikacin and cefoxitin. The biological characteristics of the strains in the main seed and working libraries were stable, and the information on passage was complete. The strain has been deposited to the National Pathogen Resource Center with preservation number.
    Conclusion The Chinese clinical S. maltophilia isolate 11066 has clear source information and typical biological and genetic characteristics of S. maltophilia. It has been evaluated according to national standard strain evaluation standards and stored in recognized source/preservation centers. It can be used as a standard/reference strain for quality control in clinical testing and basic scientific research.

     

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