刘丽, 魏强. 细菌活性检测方法研究进展及其应用探讨[J]. 凯发娱乐加盟代理, 2023, 38(12): 1519-1525. DOI: 10.3784/jbjc.202309220501
引用本文: 刘丽, 魏强. 细菌活性检测方法研究进展及其应用探讨[J]. 凯发娱乐加盟代理, 2023, 38(12): 1519-1525. DOI: 10.3784/jbjc.202309220501
Liu Li, Wei Qiang. Research progress and application of bacterial activity detection methods[J]. Disease Surveillance, 2023, 38(12): 1519-1525. DOI: 10.3784/jbjc.202309220501
Citation: Liu Li, Wei Qiang. Research progress and application of bacterial activity detection methods[J]. Disease Surveillance, 2023, 38(12): 1519-1525. DOI: 10.3784/jbjc.202309220501

细菌活性检测方法研究进展及其应用探讨

Research progress and application of bacterial activity detection methods

  • 摘要: 细菌活性检测是检验、评价和保藏的关键,快速、灵敏、特异和准确的细菌活性检测方法是十分重要的。 目前,细菌活性可以根据细菌生长繁殖能力、新陈代谢能力、细胞膜的完整性以及复制转录能力原理进行检测。不同的检测原理会有不同的检测方法,平板培养计数法是最为经典的活性检测方法,叠氮溴化丙锭结合定量聚合酶链反应(PMA-qPCR)、荧光染料结合流式细胞仪、单细胞拉曼分析技术等是新应用的方法。 随着科学技术的不断发展和细菌活性检测的需求,优化的细菌活性检测方法层出不穷,但不同的细菌活性检测方法具有不同的优缺点。 本文就目前细菌活性检测原理、方法、应用以及优缺点等方面进行描述,并探讨了细菌活性检测方法在细菌检验和保藏等方面的应用。

     

    Abstract: Bacterial activity detection is the key to inspection, evaluation and preservation. Rapid, sensitive, specific and accurate bacterial activity detection methods are very important. At present, bacterial activity can be measured according to the principles of bacterial growth and reproduction ability, metabolic ability, membrane integrity, and replication and transcription ability. Different detection principles will have different detection methods, plate culture counting method is the most classic activity detection method, Propidium Monoazide combined with Quantitative PCR(PMA-qPCR), fluorescent dye-combined flow cytometry, single-cell Raman analysis technology is the new application of instruments. With the continuous development of science and technology and the demand of bacterial activity detection, optimized bacterial activity detection methods are emerging in an endless stream, but different bacterial activity detection methods have different advantages and disadvantages. In this paper, the principle, method, application, advantages and disadvantages of bacterial activity detection were described, and the application of bacterial activity detection method in bacterial inspection and preservation was discussed.

     

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